That’s pretty much what I’m doing here. I keep finding frigging problems with my research. I hate this project. Simply hate it. First I find out a week into May that I have to redo everything that I spent the past five-six months doing because I forgot that eukaroyotes have localization sequences. Since then nothing and I mean nothing has worked. That possibility from Friday? Forget about it. My E. coli do not like my yeast protein. It seems that somehow it has been rearranged out of my farking plasmid! Yes, it’s a whole kb shorter than it should be. I’m so frustrated and stressed right now. I’ve got the dog to take care of, a new house, and myself. Plus, I’ve got to complete all this in the next few weeks. I’m beginning to doubt that this is ever going to happen.
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So sorry you’re having such a difficult time with the experiment. Honestly I can’t understand a word of what you wrote but it sounds incredibly frustrating. However I think all research goes through this stage before things start to come together. At least I hope so.
Oh, poor Amanda! I hope things get better soon.
I like your idea of not eating out. I need to resolve to do that also. How long are you trying to go? I hope you are able to work through the glitches in your research. Hang in there!
thats ass, I am sure it will work out. When this happens to me, I try working on just the first few steps, as looking at the big pic will scare the crap out of me. Good luck!
🙁 I know this feeling too well… Hang in there!
heh. i had to tell my advisor last week that i accidentally *designed a stop codon into one of my primers*. oops.
hang in there…
Just take a deep breath and break it down into bitesized chunks.
Just a thought: moving is one of the most stressful things you can go through, even when you are happy about it. Could that be coloring your perception? If so, just remember that it’s totally normal, and will pass, sooner than you think.
Oh no. Sorry that your experiments aren’t working. That happens to me every couple of months. I actually cried at my bench last month when I screwed up some samples a week before I had to give lab meeting. Somehow it all came together though. And I know your stuff will work out too. Hang in there.
I do psych, not bio-chemistry. I needed a tutor for Neuropsych because by bio background was so weak!!! (I went to high school when there were about known 16 elements!) But I sort of get what your talking about. (Never mind, it’s Greek to me!!!)
Good luck on your experiment…I’m sure this is just a stage!
Oh, I’m so sorry! I did a whole series of experiments in grad school with a plasmid containing my gene of interest before realizing I’d forgotten to put in a stop codon at the end, which meant it probably made a monster fusion protein. And I’ve had the same problem with bacteria spitting out key sequences too.
I know that doesn’t really help you any right now. I guess I’m just trying to say that you’ll find a way around these problems and it’ll work out in the end. Don’t stress too much about it–work deadlines are important, but your well-being and your life with Dr. Man and Dixie dog are more important, right? Hope things get better soon!
Fiddle 1: From June 1- 30.
Thanks everyone!
ouch, I’m sorry. Non-working experiments are so frustrating! Hope it gets better soon.