RBOC: Lab Work Edition

I don’t have enough wherewithal to give you an actual post. So, I’ve decided to just give you snippets of things I’m thinking about.

  • I’m finally feeling better! I no longer snot all over the lab. CurrentLabMate (formerly known as FormerRoommate, unfortunately I’m just not that creative today) is appreciative that I’ve stopped doing that. I’m slightly behind in lab, as I took people’s advice and went home early on Thursday and Friday. Also, I didn’t work (much) this past weekend. That last bit is probably why I feel so much better now.
  • Undergrads today just have no idea how to get information (yes, I know. I, also, yell at kids to get off my lawn). I’m TAing UndergradBiochem (I use the term TA loosely. Mostly I just hold office hours and proctor exams) and I have had several emails asking me about how Gibb’s Free Energy and Allosteric Inhibitors work. Seriously people, look it up in your textbook. I don’t have some magical new answer for you. Trust me, Gibb’s hasn’t changed since I was in your shoes and there’s always the same equation. Even if you don’t own the textbook (sigh, it’s a book, it’s required, and you’ll need it in med school), there’s always Google.
  • Another random complaint about the undergrads. Please don’t ask, “Is this going to be on the exam?” Because (1) it’s annoying and (2) I don’t write the exam. I’ve told you this multiple times. The person to ask would be the actual professor.
  • I’ve learned to multi-task(ish). Previously, I’ve had this experiment that I’d like to do, but it takes many hours over many days. My first thought was to put it off until I could block out a period of time. It dawned on me (recently) that it’d make much more sense to just cram it into my days and deal. That’s what I’ve been doing for the past month and, wonders of wonders, I’ve made really good progress. It’s not done, but I’ve gotten through some of the trickier parts. So, I’m fairly pleased about that.
  • Dr. Man and I have actually started scheduling time to spend together. I know, it’s really lame. And I feel very lame. But between his residency and my lab work, it was getting to the point to where we only saw each other when we were burnt out husks of people. So, now we’ve got one night a week where we both leave at a decent time, go home, have dinner, talk, and watch a movie. So far, it’s gone pretty well. Eventually, we may not even have to schedule time to be together. Wouldn’t that be nice?
Posted in Random | 9 Comments

Sick-ish

I’ve been sick-ish the past couple of days. Sick-ish in the sense that I haven’t been running a fever, but I’ve had the congested head, cough, sore throat, and general aches/pains of being sick. I freely admit that I’m a wuss when it comes to being sick. I don’t like anything that makes me feel bad. So, I complain and whine. I talk about my symptoms like I’m the only person on the planet to ever have a cold and that no one can ever feel my pain. Well, at least that’s what I say in my head. Mostly I know that sort of behavior is really obnoxious, so I keep it on the inside. Still, I think it and thats almost as good as saying it out loud except that it annoys Dr. Man much less.

Knowing all this, I also have a hard time deciding to stay home. There’s all this stuff to be done in the lab and it’s actually kind of fun stuff. It’s new and different. It may lead to at least preliminary evidence on my working hypothesis. Those sort of experiments are the ones that made me decide to become a scientist. That means that I want to go into the lab. And yet… I feel so horrible that all I want to do is crawl into bed, pull the covers up, and sleep. Then again, when I have little sleep I feel the same way. So, I now have the fever rule. If I’m sick enough to run a fever, then I’m sick enough to stay home. No fever, then I head into work (and take enough medicine to start a meth lab in my belly).

Hence, I’ve been in the lab this week. I’ve gone through the majority of a box of kleenex, an entire box of sudafed, and half a bottle of ibuprofen. However, I’ve troubleshot my protein purification, found a way to get more protein, contacted a collaborator, and made minor progress on Damned Yeast Project. Also, I’ve provided entertainment for the lab. They’ve set up a pool based on the number of times I wash my hands in one day. I think the loser has to load pipet tips.

Posted in Grad School | 12 Comments

Lecture Update

I’ve been horrible about updating. And it’s for the usual reasons– I’m very, very busy. In the past two weeks I have: presented to the department for journal club, given the lecture to Advisor’s class, and held a committee meeting. They all went reasonably well– at least so I’ve been told. And now I’m sick with a lovely drippy nose, stuffy head, and sore throat. And for some reason I’m still plugging away in the lab. Sigh.

First, I wanted to thank everyone for their suggestions on my lecture. It went pretty well. Advisor thought that the troubleshooting section I went through was a great idea and a good way to present the information! (Thanks, everyone!) The thing that surprised me the most was how much time it took to prepare the lecture. I was doing it on a technique I use frequently and it took hours. I think I spent more time preparing for this one lecture than I’ve spent studying any one lecture in my entire life. I really didn’t want to screw anything up and say something blatantly wrong, thus placing the wrong idea into a student’s head and scarring them for life. So, I might have over prepared.

I also found myself having to think on my feet a bit, when having to explain something more to a student (There were a few times when someone asked me to go back over a slide. I’d try to come up with another way to describe the slide because I figured it didn’t make any sense to just repeat what I said if they didn’t get it the first time or I’d try to expand upon whatever part they didn’t understand.). However, I found that I liked doing that. It felt like I wasn’t just talking at them and I liked the part when they’d suddenly understand (or at least they’d fake it well enough). It also helped me realize things that I didn’t explain very well.

This little venture also led me into Test Question Writing Land. That I don’t know if I did all that too terribly well on. Advisor said that it’s a fair, but open question (as in it’s not a Define This Term or Multiple Choice type of question, it’s a Describe Method X type question). I think it might’ve been too open as the answers vary wildly in content and quality. I’m taking that into account with the grading. I think grading may be best done with a beer in hand, though.

Posted in Trying my hand at teaching | 4 Comments

Messages

In my quest to get not just data but actual results, I’m spending some quality time with my enzyme today. (I usually only work Sundays as part of my new-resolution to make my work week bearable. But that’s another blog post.) Anyhow, I crept out of bed this morning while Dr. Man slumbered away and went to the lab. I’ve been here ever since. I received a phone call around 11:30 asking about lunch. I told him that I was stuck here until at least 1pm (sigh, a watched column never produces any flow through). We talked for a little bit longer and he asked me about lunch. I told him not to wait and we hung up. About an hour later, I receive a text message. I see that it’s from him and I smile thinking that it’ll be some nice, sweet message expressing his undying love and affection or expressing sympathy that I’m working. But, no, I get “Can u buy beer?”

At least my working today wasn’t for naught. I can bring home the bacon… er… beer.

Posted in Uncategorized | 6 Comments

Blank Screen Anxiety

It seems that I have blank screen anxiety with everything right now. I’ve been thinking about writing a blog post, but I haven’t been able to write anything that seems (1) profound or (2) even just a bit entertaining. Or (3) I’ve been consumed with anxiety about writing something that I think is somewhat entertaining, but really isn’t to anyone else who doesn’t live inside my head (which, frankly, is a valid concern). Another idea that has been running through my mind is that I have been edging towards whining a lot here and I should stop that.* Whining isn’t really going to help anyone– unless it’s funny, but that puts me back to some-what-valid-concern #3. To make matters even more convoluted is that the busier I am, the more I want to whine and the more I want to whine the more I want to blog and so it goes (apparently in run-on sentence form). To break the cycle, I figured I should just post something– anything really– and just write.

This blank screen anxiety seems to have been brought on by the dreaded, twice-yearly committee meeting and the progress report that it entails. The committee meeting itself isn’t really all that too terribly traumatizing. It’s just that I don’t think I have enough data to make one necessary. Since my last committee meeting (in February) I have done:
– Submitted abstract to Major European Meeting
– Shown poster at Major European Meeting (this shows only a moderate amount of progress on Damned Yeast Project)
– Made approximately two major design mistakes (one of which I will share the blame with Advisor and, luckily, he admits to some blame)
– Made hardly any progress on Interesting Technique Project That Requires Fancy Piece of Equipment
And that’s about that. I have some great data for troubleshooting, but nothing new and exciting. Mostly just along the lines of “Yep, tried this. But I found out it didn’t work because of X. So, I tried Y next and that isn’t going to work because of Z.”

I have a clear plan on where to go from here (basically, I was able to design a new plan to surmount problems X and Z and I’m getting better at multi-tasking). It’s just that implementing this plan since I’ve gotten back has been rather slow going because workaround L took a good long amount of time and that yeast just grow slower than bacteria (seriously, much slower– it’s driving me up a wall). And my ability to multi-task on projects recently has been rather abysmal. I tend to focus a lot on one particular project and I follow it through until I’m done. The only problem with that is all the other projects I need to do at the same time suffer. Especially since my Interesting Technique project has fallen off Advisor’s radar and it’s easier for me to focus on what he considers urgent.

Ultimately, though, my lack of progress over the past six months really is my fault. More should have gotten done and it’s my fault that it hasn’t. The mistakes that I made are my fault. What, I suppose, it comes down to is that I already feel badly about screwing things up and I’m working twice as hard to fix them. So, I’m dreading more people telling me that I’ve failed. I can make myself feel that way enough on my own. I guess I should just hope that I don’t screw things up as much in the next six months, huh?

* This post is still me whining. But I’d liken it more to verbal diarrhea. A fine distinction, but a distinction nonetheless.

Posted in Uncategorized | 8 Comments

Notes, Lectures, and Students! Oh my!

I have to give a lecture and I’m ridiculously worried about it. Well, worry may not be the correct word. What’s actually going through my mind is: How am I going to fill up 45 mins?

Since my epiphany about wanting a family and not necessarily wanting to work 70+ hour weeks for forever, I’ve been looking into alternative careers in Science and variations on the R1-institution-professorship thing (like maybe SLAC or something?) that’s pushed at Public U. I (and probably you) already know that writing is not my thing. Words don’t flow, grammar is a mystery, and it’s, well, not my thing. That means that I’m leaning away from a career in science writing. I like the idea of a position like Mad Hatter’s position. I also like the idea of teaching and doing research at a smaller-scale institution (as Public U. is huge). So, what does this have to do with anything? Well, I’ve never given a lecture… ever.

In my particular program we don’t have a lot of teaching opportunities. There’s TAing the undergrad biochem course and some med/dental school biochem/lab courses. However, we don’t lecture in those courses. TAing consists of office hours and proctoring exams. All I’ve gotten experience in is tutoring, sitting and watching for cheaters, and supervising undergrads. Still, no real teaching per se.

I was discussing this with Advisor (not the whole what-am-I-doing-with-my-life thing, but the interest in teaching thing) and he offered to let me give a lecture in his course (and do the review session that’s a part of the class and a test question). The lecture should be fairly easy. It’s a technique lecture and it’s a technique that I use a lot. It’s an easy technique and relatively short. Hence, the time-filling problem. Plus, I’m trying to pitch it to the right level (it’s a course for first-year masters students). As in PCR should be a known concept, but do I explain what makes a good primer? Do I go over the history of the technique? And can I use my own (published) data to explain the technique or is that just arrogant (I do have the figures already made)?

Ok, I’ll get back to work now. I’m sure I’m making a big deal out of nothing… right?

Posted in Grad School | 8 Comments

Why I hate Meeting/Seminar Filled Days

Warning Rant Ahead:

There’s this one day of my week that is totally shot. I’ve tried and tried to be able to do something in the lab on this day of the week, but it’s impossible. Or at least impossible without not showing up to some required thing. We have journal club over lunch, then I have office hours, and then there’s seminar. It used to be that the latter wasn’t required. So, I’d attend the (required) journal club and then hold office hours (which, while a pain, are useful in the monetary department). Then, I’d skip the seminar so I could salvage something out of this day. But now it is required. So, for five hours I’m stuck outside of the lab.

This wouldn’t be so bad if I didn’t have to pay for it the rest of the day and the day before and after. I get in early, leave late, do work at home and I still have more things to do than time in the following day.

Good I’m glad that I got that out.

Posted in Rant, whining | 9 Comments

What I did over summer…er… semester

I didn’t get much a vacation this summer. I spent the first three quarters of it frantically working to have something to present at Major European Conference. Only to find out that I have to work twice as hard on Damned Yeast Project to get it out the door sooner rather than later (so that break after working nonstop was going to have to wait). So, instead of posting about working X hour days (where X>9), I’m going to focus on the second best part of the summer (the best part being when Dr. Man moved back home– I know, sickeningly sweet). Without further ado, the (second) best part of my summer was: the week I took off to spend in the Emerald Isle and Nearby Country.

As part of the Major European conference, I stayed here:

Well, perhaps not quite here, but on the same campus.

I also made it out to the country side.

Sheep!

I was amazed by the amount of sheep. And the amount of scat they produced. The area where I took this picture was littered with it. I had to carefully watch my step. My Brother was not so lucky, though, and found that he had not tread carefully enough.

Building a henge are we?

I was rather disappointed with this place. I thought that it’d be in the middle of nowhere and perhaps I’d have to answer about the airspeed of a swallow in order to see it. However, it was sandwiched between two major highways and there were a. lot. of. people. So, not quite the mystical experience I thought it’d be.

****
I think the rest of the summer can be summarized into these two sentences:
(1) Do not assume that Advisor knows more about this stuff than I do.
(2) Think every experiment through. In other words: Don’t Panic.

Posted in scientiae-carnival | 5 Comments

Amanda’s Guide to Extreme Frugality: Food Edition

Disclaimer: I did not follow all of these things all of the time, but I try to most of the time. I’ve also added in somethings that I’ve learned about recently. Oh, and additional ideas/tips are welcome in the comments.

Dr. Man and I got married after his second year of med school and before my first year of grad school. This meant for the first two years of our marriage I was the only one with any positive income (by positive, I mean opposed to negative– as in loans). In order to minimize the number of loans we had to pull out (med school is expensive!), we learned to live frugally and I learned some extreme frugality tips. So, I figured that I’d share with everyone else. The key to maintaining this is not to try to adopt all the frugality tips at once, but to ease yourself into it. Also, pick the ones that work for you. So, on to the guide.

In this part I’ll focus on Food:

-Do NOT pick frozen dinners just because they are cheap. They will end up costing more than if you just made the dinner yourself and froze the leftovers for lunch.
-Repeat after me, Sales Are Your Friend. This means that whenever pasta and pasta sauce goes on sale– stock up. Pasta/Pasta Sauce was a main staple for Dr. Man and I because we both liked it and could jazz it up in a variety of ways.
-Additionally, whenever ground meat (if you are carnivorous) goes on sale buy a lot. Then, split the packages into ~1/2 lbs packs and freeze. This is about right for making a meal for yourself with enough leftover for lunch or dinner.
-A second part to this is to take advantage of the internet. A lot of grocery stores post their weekly fliers online. So, take a look there to figure out where the best deals are at for the week.
-I went so far as to make a “grocery log” where I charted the prices of our staple food (flour, milk, butter, etc.). Pretty soon you’ll notice that the grocery store price things in cycles.
-Make your own bread. It’s much cheaper and delicious.
-Powdered milk. I don’t like to drink it on its own, but as far as baking, cooking, or making instant pudding it’s delicious.
-Beans, beans, the musical fruit… well it may make you more musically-inclined, but beans are an excellent food source. A large bag of Fifteen Bean Soup (I’m talking serving size of approx. 14 here) is around $2 at Grocery Store here. And it comes with everything you need to make said soup. That works out to be about 15 cents a serving!
-Now the trickiest part of food shopping: Fruits and Vegetables. To this I say, “Get thee to thy Local Farmer’s Market.” Seriously, this is how Dr. Man and I afforded fresh fruits and veggies. Go towards the end of the scheduled time (I’d say somewhere between 15-30 mins before the market is scheduled to end) and haggle with the sellers. Don’t try to haggle with anyone who has their own trailer in sight (unless you like to do things the hard way or you like an exercise in futility), but try those that have a limited amount of produce left or that are parked far away from their stand. A lot of the time you can get their produce for anywhere between 25%- 50% off just so they don’t have to cart it back to their car/truck/etc. Plus if you live in a college town and plead poor grad student, sometimes you’ll get extra stuff thrown in. And (at least at our Farmer’s Market) the produce is already just a little bit cheaper than at Grocery Store.
-Booze. As any graduate student knows, alcohol is a very important food group. So, these are the things that I’ve found to help lower the cost of such things. Sam’s Club (or any whole-sale club) usually has a discount liquor store– with cut-rate beer and wine. Make friends with your local, independent liquor store clerk. They’ll point you towards good deals and cheap, but tasty wine (ok, I like wine). Or if you have a clearance liquor store (our Chain Liquor Store has one a few miles from Public U.), go there and find stuff you like on the cheap.

Stay tuned for the next installment…

Posted in Uncategorized | 9 Comments

Education Fail

My cousin is at university now. My university. It’s definitely a little strange that someone I remember being born is now old enough to go to college. As someone who has been at Public U. for approximate 65 million years (it was just me and the dinosaurs– no bureaucrats and it was bliss), I decided to show her around. I took her to the various buildings her classes would be in and answered her questions about what to bring to class, where to buy textbooks, and where to buy beer (Answers: pen and paper and no books, half.com or Independent Cheap Bookstore, and no where, you’re underage young lady). Then, we passed by a group of Young Political Party registering people to vote. I am a procrastinator and I have yet to change my voter’s registration address. So, I seized the opportunity to change my address. Cousin decided to change her address as well. As we were leaving she asked me who I liked for the election. I told her Obama. She said that she did, too, but that she was an idiot and registered Republican and that meant she had to vote for McCain.

Sigh.

Posted in Uncategorized | 9 Comments